Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Bacillus (tested or 100% immunogen sequence identity)
ELISA (1:7500 - 1:32000)
Specificity and Use
Glucose Dehydrogenase [Bacillus].
Glucose Dehydrogenase [Bacillus]. Cross reactivity against Glucose Dehydrogenase from other sources is unknown
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of Glucose Dehydrogenase [Bacillus] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) catalog no. LS-C60884 and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Glucose Dehydrogenase Polyclonal Antibody-Western blot. Goat anti-Glucose Dehydrogenase antibody (LS-C59219 lot 6454) was used to detect purified Glucose Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti-goat (1:10K in TBS/MB-070 1 hr RT). Images were collected using the Bio-Rad VersaDoc System. This image was taken for the unconjugated form of this product. Other forms have not been tested.