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Anti-GLS2 / Glutaminase 2 Antibody (aa241-290) LS-C80586

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl 1 mg/ml Unavailable

Most Popular GLS2 / Glutaminase 2 Antibodies

Anti-GLS2 / Glutaminase 2 Antibody (aa241-290) LS-C80586
Rabbit Polyclonal (IgG) to Human GLS2 / Glutaminase 2
Human, Mouse, Rat, Bat, Dog, Horse, Pig, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GLS2 / Glutaminase 2 Antibody (aa498-524) LS-C166518
Rabbit Polyclonal to Human GLS2 / Glutaminase 2
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GLS2 / Glutaminase 2 Antibody LS-C364669
Rabbit Polyclonal to Human GLS2 / Glutaminase 2
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human GLS2 / Glutaminase 2
Human, Mouse, Rat, Bat, Dog, Horse, Pig, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human GLS2 / Glutaminase 2
Rabbit
Human, Mouse, Rat, Bat, Dog, Horse, Pig, Zebrafish (tested or 100% immunogen sequence identity)
Monkey, Bovine, Guinea pig, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

GLS2 / Glutaminase 2 antibody was raised against synthetic peptide located between aa241-290 of human GLS2 (Q9UI32). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bat, Horse, Pig, Opossum (100%); Marmoset, Bovine, Rabbit, Guinea pig (92%).
aa241-290
Human GLS2 / Glutaminase 2
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 2.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.Antibody has been tested in IHC using formalin-fixed paraffin-embedded human tissues only.

Packaging

Lyophilized from PBS with 2% sucrose
100 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GLS2 / Glutaminase 2

Q9UI32 NM_013267 NP_037399.2

GLS2 Antibody, GA Antibody, Glutaminase 2 Antibody, Glutaminase I Antibody, Glutaminase GA Antibody, L-glutaminase Antibody, HLGA Antibody, L-glutamine amidohydrolase Antibody, LGA Antibody, Breast cell glutaminase Antibody

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.

Immunohistochemistry

Immunohistochemistry
GLS2 / Glutaminase 2 antibody LS-C80586 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 3/29/2017

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