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Antibodies > GLS / Glutaminase > LS-C166517
 
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Catalog Number Size Price
LS-C166517-200 200 µl (0.5 mg/ml) $393 
GLS / Glutaminase Antibody - GLS Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GLS / Glutaminase Antibody - Immunohistochemical of paraffin-embedded H.kidney section using GLS Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
GLS / Glutaminase Antibody - Confocal immunofluorescence of GLS Antibody with HeLa cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
GLS / Glutaminase Antibody - Fluorescent image of HeLa cells stained with XAF1 GLS Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
GLS / Glutaminase Antibody - Fluorescent image of HeLa cells stained with XAF1 GLS Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
GLS / Glutaminase Antibody - Western blot of lysate from HeLa cell line, using GLS Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
GLS / Glutaminase Antibody - Western blot of lysates from 293, 293T, HeLa cell line , human brain and mouse brain tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
GLS / Glutaminase Antibody - Western blot of GLS Antibody in mouse liver tissue lysates (35 ug/lane). GLS (arrow) was detected using the purified antibody.
GLS / Glutaminase Antibody - Western blot of lysates from human brain and mouse brain tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
GLS / Glutaminase Antibody - Western blot of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
GLS / Glutaminase Antibody - Western blot of lysates from 293T, HeLa cell line (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
GLS / Glutaminase Antibody - GLS Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GLS / Glutaminase Antibody - GLS Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GLS / Glutaminase Antibody - Immunohistochemical of paraffin-embedded H.kidney section using GLS Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
GLS / Glutaminase Antibody - Confocal immunofluorescence of GLS Antibody with HeLa cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
GLS / Glutaminase Antibody - Fluorescent image of HeLa cells stained with XAF1 GLS Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
GLS / Glutaminase Antibody - Fluorescent image of HeLa cells stained with XAF1 GLS Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
GLS / Glutaminase Antibody - Western blot of lysate from HeLa cell line, using GLS Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
GLS / Glutaminase Antibody - Western blot of lysates from 293, 293T, HeLa cell line , human brain and mouse brain tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
GLS / Glutaminase Antibody - Western blot of GLS Antibody in mouse liver tissue lysates (35 ug/lane). GLS (arrow) was detected using the purified antibody.
GLS / Glutaminase Antibody - Western blot of lysates from human brain and mouse brain tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
GLS / Glutaminase Antibody - Western blot of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
GLS / Glutaminase Antibody - Western blot of lysates from 293T, HeLa cell line (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
GLS / Glutaminase Antibody - GLS Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human GLS / Glutaminase Antibody (aa516‑545, IHC, IF, WB) LS‑C166517

Polyclonal Rabbit anti‑Human GLS / Glutaminase Antibody (aa516‑545, IHC, IF, WB) LS‑C166517

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Antibody:
GLS / Glutaminase Rabbit anti-Human Polyclonal (aa516-545) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C166517-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GLS / Glutaminase Rabbit anti-Human Polyclonal (aa516-545) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Glutaminase antibody LS-C166517 is an unconjugated rabbit polyclonal antibody to Glutaminase (GLS) (aa516-545) from human. It is reactive with human, mouse and rat. Validated for Flow, IF, IHC and WB.
Target
Human GLS / Glutaminase
Synonyms
GLS | AAD20 | GLS1 | GAM | Glutaminase C | GAC | K-glutaminase | KIAA0838 | L-glutamine amidohydrolase | Glutaminase | KGA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa516-545
Specificity
This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GLS / Glutaminase
O94925 NM_014905 NP_055720.3

Publications (0)

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Featured Products

GLS / Glutaminase Antibody - GLS antibody Western blot of ACHN lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLS / Glutaminase Antibody (C‑Terminus) LS‑C111602
Species: Human, Rat
Applications: Western blot
GLS / Glutaminase Antibody - Western Blot; Sample: Human 293T cell lysate; Primary Ab: 2µg/ml Rabbit Anti-Mouse GLS Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
GLS / Glutaminase Antibody (aa31‑253, PE) LS‑C728142
Species: Mouse, Human
Applications: Western blot
GLS / Glutaminase Antibody - Immunofluorescence of monoclonal antibody to GLS on HeLa cell. [antibody concentration 10 ug/ml]
GLS / Glutaminase Antibody (clone 5C4) LS‑C197311
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Product
GLS / Glutaminase Antibody (aa516‑545, FITC) LS‑C244465
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
GLS / Glutaminase Antibody - All lanes: Anti-GLS Antibody (N-Term) at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 73 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
GLS / Glutaminase Antibody (aa144‑177) LS‑C392911
Species: Human, Mouse
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
GLS / Glutaminase ELISA Kit
Mouse GLS / Glutaminase (Sandwich ELISA) ELISA Kit - LS-F6990
Reactivity: Mouse
Range: 0.313-20 ng/ml
See all GLS / Glutaminase Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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