LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-GLI3 Antibody (aa1-479) LS-C150112

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GLI3 Antibodies

Anti-GLI3 Antibody (aa41-57) IHC-plus™ LS-B73
Rabbit Polyclonal to Human GLI3
Human, Dog, Chicken, Xenopus, Quail, Squirrel monkey
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GLI3 Antibody (aa1-479) LS-C150112
Goat Polyclonal (IgG) to Human GLI3
Human, Mouse
ICC, Western blot, Immunoprecipitation
Unconjugated
Immunocytochemistry Image
Anti-GLI3 Antibody (aa975-1025) LS-C290096
Rabbit Polyclonal (IgG) to Human GLI3
Human
Western blot, Immunoprecipitation
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human GLI3
Human, Mouse
ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human GLI3
Goat
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Immunoprecipitation

Specificity and Use

GLI3 antibody was raised against e. coli-derived recombinant human GLI3 Met1-Glu479 Accession # P10071.
aa1-479
Detects human and mouse GLI3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with.
Western Blot, 1 µg/ml, Chromatin Immunoprecipitation (ChIP), 5 µg/106 cells. Immunocytochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About GLI3

P10071 NM_000168 NP_000159.3

GLI3 Antibody, ACLS Antibody, GCPS Antibody, GLI-Kruppel family member GLI3 Antibody, GLI3 full length protein Antibody, GLI3-190 Antibody, GLI family zinc finger 3 Antibody, Gli-3 Antibody, GLI3FL Antibody, PAP-A Antibody, PAPA1 Antibody, PHS Antibody, PPDIV Antibody, Transcriptional activator GLI3 Antibody, PAPA Antibody, PAPB Antibody, GLI3 form of 190 kDa Antibody, Oncogene GLI3 Antibody, Zinc finger protein GLI3 Antibody

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor.

Immunocytochemistry

Immunocytochemistry
GLI-3 in HeLa Human Cell Line. GLI-3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).

Western blot

Western blot
Detection of Mouse GLI3 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human/ Mouse GLI3 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for GLI 3 at approximately 85 kD (as indicated). This experiment was conducted under reducing conditions.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Detection of GLI-3-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-3/DNA complexes were immunoprecipitated using 5 ug Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The GLI-1 promoter was detected by standard PCR.
 

Requested From: United States
Date Requested: 12/9/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number