Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
GLI3 Antibody, ACLS Antibody, GCPS Antibody, GLI-Kruppel family member GLI3 Antibody, GLI3 full length protein Antibody, GLI3-190 Antibody, GLI family zinc finger 3 Antibody, Gli-3 Antibody, GLI3FL Antibody, PAP-A Antibody, PAPA1 Antibody, PHS Antibody, PPDIV Antibody, Transcriptional activator GLI3 Antibody, PAPA Antibody, PAPB Antibody, GLI3 form of 190 kDa Antibody, Oncogene GLI3 Antibody, Zinc finger protein GLI3 Antibody
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor.
GLI-3 in HeLa Human Cell Line. GLI-3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).
Detection of Mouse GLI3 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human/ Mouse GLI3 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for GLI 3 at approximately 85 kD (as indicated). This experiment was conducted under reducing conditions.
Detection of GLI-3-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-3/DNA complexes were immunoprecipitated using 5 ug Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The GLI-1 promoter was detected by standard PCR.