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Anti-GLI3 Antibody (aa1-479) LS-C150112

Catalog Size Price
LS-C150112-25 25 µg Unavailable
LS-C150112-100 100 µg Unavailable

Related Products

16 GLI3 Antibodies

Most Popular GLI3 Antibodies

Anti-GLI3 Antibody (aa41-57) IHC-plus™ LS-B73
Rabbit Polyclonal to Human GLI3
Human, Dog, Chicken, Xenopus, Quail, Squirrel monkey
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image
Anti-GLI3 Antibody (aa1-479) LS-C150112
Goat Polyclonal (IgG) to Human GLI3
Human, Mouse
ICC, Western blot, Immunoprecipitation
Immunocytochemistry Image
Anti-GLI3 Antibody (aa975-1025) LS-C290096
Rabbit Polyclonal (IgG) to Human GLI3
Western blot, Immunoprecipitation
Anti-GLI3 Antibody (aa303-561) LS-C294190
Rabbit Polyclonal (IgG) to Mouse GLI3
Western blot
Western blot Image
Anti-GLI3 Antibody (aa30-110) LS-C383698
Rabbit Polyclonal (IgG) to Human GLI3
Human, Mouse, Rat
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100% Guaranteed
Goat Polyclonal (IgG) to Human GLI3
Human, Mouse
ICC, Western blot, Immunoprecipitation


Human GLI3
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Immunoprecipitation

Specificity and Use

GLI3 antibody was raised against e. coli-derived recombinant human GLI3 Met1-Glu479 Accession # P10071.
Detects human and mouse GLI3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with.
Western Blot, 1 µg/ml, Chromatin Immunoprecipitation (ChIP), 5 µg/106 cells. Immunocytochemistry, 5-15 µg/ml.


Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About GLI3

P10071 NM_000168 NP_000159.3

GLI3 Antibody, ACLS Antibody, GCPS Antibody, GLI-Kruppel family member GLI3 Antibody, GLI3 full length protein Antibody, GLI3-190 Antibody, GLI family zinc finger 3 Antibody, Gli-3 Antibody, GLI3FL Antibody, PAP-A Antibody, PAPA1 Antibody, PHS Antibody, PPDIV Antibody, Transcriptional activator GLI3 Antibody, PAPA Antibody, PAPB Antibody, GLI3 form of 190 kDa Antibody, Oncogene GLI3 Antibody, Zinc finger protein GLI3 Antibody

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor.


GLI-3 in HeLa Human Cell Line. GLI-3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).

Western blot

Western blot
Detection of Mouse GLI3 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human/ Mouse GLI3 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for GLI 3 at approximately 85 kD (as indicated). This experiment was conducted under reducing conditions.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Detection of GLI-3-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-3/DNA complexes were immunoprecipitated using 5 ug Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The GLI-1 promoter was detected by standard PCR.

Requested From: 
Date Requested: 3/24/2018

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