LSBio The Immunohistochemistry Antibody Company
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-GLI2 Antibody LS-C149960


Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular GLI2 Antibodies

Anti-GLI2 Antibody (Internal) IHC-plus™ LS-B75
Rabbit Polyclonal to Mouse GLI2
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-GLI2 Antibody (aa46-60) IHC-plus™ LS-B2808
Rabbit Polyclonal to Human GLI2
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-GLI2 Antibody (aa1287-1321) LS-C344252
Rabbit Polyclonal to Human GLI2
Immunofluorescence, Western blot, Flow Cytometry
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human GLI2
IHC - Paraffin, Western blot, Immunoprecipitation


Human GLI2
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Immunoprecipitation

Specificity and Use

GLI2 antibody was raised against e. coli-derived recombinant human GLI2 Ala2-Glu91 Accession # BAA25668.
Detects human GLI2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with.
Western Blot, 0.1 µg/ml, Recombinant Human GLI-2. Chromatin Immunoprecipitation (ChIP), 5 ug/5 x 106 cells. Immunohistochemistry, 5-15 µg/ml, Immersion fixed paraffin-embedded sections of human skin, basal cell carcinoma, and. prostate cancer tissue.


Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About GLI2

P10070 NM_005270 NP_005261.2

GLI2 Antibody, GLI-Kruppel family member GLI2 Antibody, HPE9 Antibody, Gli-2 Antibody, Oncogene GLI2 Antibody, Tax helper protein 2 Antibody, THP Antibody, Zinc finger protein GLI2 Antibody, Tax helper protein Antibody, THP2 Antibody, GLI family zinc finger 2 Antibody, Tax helper protein 1 Antibody, THP1 Antibody

GLI2 is a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Detection of GLI-2-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-2/DNA complexes were immunoprecipitated using 5 ug Sheep Anti-Human GLI-2 Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The bcl-2 promoter was detected by standard PCR.

Requested From: United States
Date Requested: 10/22/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number