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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GLI2 Antibody (aa600‑649) LS‑C31484
GLI2 antibody LS-C31484 is an unconjugated rabbit polyclonal antibody to human GLI2. Validated for IHC and WB.
Catalog
Size
Price
LS-C31484-100
100 µg (1 mg/ml)
Unavailable

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Product Description

GLI2 antibody LS-C31484 is an unconjugated rabbit polyclonal antibody to human GLI2. Validated for IHC and WB.
About GLI2
GLI2 is a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. P10070 NM_005270 NP_005261.2

GLI2 Antibody, GLI-Kruppel family member GLI2 Antibody, HPE9 Antibody, Gli-2 Antibody, Oncogene GLI2 Antibody, Tax helper protein 2 Antibody, THP Antibody, Zinc finger protein GLI2 Antibody, Tax helper protein Antibody, THP2 Antibody, GLI family zinc finger 2 Antibody, Tax helper protein 1 Antibody, THP1 Antibody


Specifications

Target
Human GLI2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1.25 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GLI2 antibody was raised against synthetic peptide located between aa600-649 of human GLI2 (P10070). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%); Monkey (92%).
Epitope
aa600-649
Specificity
Human GLI2
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
100 µl After adding water, will consist of PBS buffer with 2% sucrose
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human heart.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human heart. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human heart.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human heart. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human heart.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human heart. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human heart.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human heart. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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