Human GLI2

Rabbit

Human, Chimpanzee (tested or 100% immunogen sequence identity)

Chimpanzee (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human Gli-2 (isoform a).

A BLAST analysis was used to suggest cross reactivity with Gli-2 from human and chimpanzee based on the immunizing sequence.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:500 - 1:2000)
• ELISA (1:10000 - 1:30000)

Immunohistochemistry: LS-B2808 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2808 was determined to be 5 ug/ml. Western Blot: Multiple splice variants have been reported for this protein. Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10070 NCBI: NM_005270 NP_005261.2