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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GLI / GLI1 Antibody (DY550) LS‑C180831
GLI1 antibody LS-C180831 is a DY550-conjugated rabbit polyclonal antibody to GLI1 (GLI) from human and mouse. Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C180831-100
100 µl (1.08 mg/ml)
Unavailable

Popular GLI / GLI1 Products

Anti-GLI1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7244 concentration 5 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Chromatin Immunoprecipitation
Immunohistochemistry: Gli1 Antibody - IHC of Gli1 in mouse brain using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot
IHC of paraffin-embedded Human skin tissue using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

GLI1 antibody LS-C180831 is a DY550-conjugated rabbit polyclonal antibody to GLI1 (GLI) from human and mouse. Validated for ICC, IHC and WB.
About GLI / GLI1
Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. P08151 NM_005269 NP_005260.1

GLI1 Antibody, Gli-1 Antibody, GLI family zinc finger 1 Antibody, Glioma-associated oncogene Antibody, Glioma-associated oncogene 1 Antibody, Oncogene GLI Antibody, GLI Antibody, Zinc finger protein GLI1 Antibody


Specifications

Target
Human GLI / GLI1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
DyLight 550. Also available Unconjugated or conjugated with Biotin, DY650, DY488.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:400)
  • ICC (1:200)
  • Western blot (1:1000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GLI / GLI1 antibody was raised against a synthetic peptide made to an internal portion of the human Gli1 protein (between residues 150-200) [UniProt P08151].
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C in the dark.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunohistochemistry: Gli1 Antibody - IHC of Gli1 in mouse brain using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: Gli1 Antibody - IHC of Gli1 in mouse brain using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: Gli1 Antibody - Gli1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: Gli1 Antibody - Gli1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: Gli1 Antibody - WB analysis of Gli1 on partial recombinant Gli1 protein (molecular weight of partial recombinant protein is 37 kDa).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: Gli1 Antibody - WB analysis of Gli1 on partial recombinant Gli1 protein (molecular weight of partial recombinant protein is 37 kDa). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: Gli1 Antibody - IHC of Gli1 in mouse brain using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: Gli1 Antibody - IHC of Gli1 in mouse brain using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: Gli1 Antibody - Gli1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: Gli1 Antibody - Gli1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: Gli1 Antibody - WB analysis of Gli1 on partial recombinant Gli1 protein (molecular weight of partial recombinant protein is 37 kDa).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: Gli1 Antibody - WB analysis of Gli1 on partial recombinant Gli1 protein (molecular weight of partial recombinant protein is 37 kDa). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: Gli1 Antibody - IHC of Gli1 in mouse brain using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: Gli1 Antibody - IHC of Gli1 in mouse brain using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: Gli1 Antibody - Gli1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: Gli1 Antibody - Gli1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: Gli1 Antibody - WB analysis of Gli1 on partial recombinant Gli1 protein (molecular weight of partial recombinant protein is 37 kDa).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: Gli1 Antibody - WB analysis of Gli1 on partial recombinant Gli1 protein (molecular weight of partial recombinant protein is 37 kDa). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: Gli1 Antibody - IHC of Gli1 in mouse brain using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: Gli1 Antibody - IHC of Gli1 in mouse brain using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: Gli1 Antibody - Gli1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: Gli1 Antibody - Gli1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot: Gli1 Antibody - WB analysis of Gli1 on partial recombinant Gli1 protein (molecular weight of partial recombinant protein is 37 kDa).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot: Gli1 Antibody - WB analysis of Gli1 on partial recombinant Gli1 protein (molecular weight of partial recombinant protein is 37 kDa). This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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