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Anti-GLI / GLI1 Antibody (clone 388516) LS-C150111

Ordering

Wt. Vol. Conc. Price
100 µg - - $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GLI / GLI1 Antibodies

Anti-GLI / GLI1 Antibody LS-C180157
Rabbit Polyclonal to Human GLI / GLI1
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GLI / GLI1 Antibody (aa755-1106, clone 1C1) LS-C339074
Mouse Monoclonal [clone 1C1] (IgG1) to Human GLI / GLI1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GLI / GLI1 Antibody (aa755-1106, clone 4E2) IHC-plus™ LS-B13058
Mouse Monoclonal [clone 4E2] (IgG1) to Human GLI / GLI1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone 388516] (IgG2a) to Human GLI / GLI1
Human, Mouse
ICC, Western blot
Unconjugated

Details

Human GLI / GLI1
Rat
Human, Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [388516]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • ICC (8 - 25 µg/ml)
  • Western blot (0.1 µg/ml)

Specificity and Use

GLI / GLI1 antibody was raised against e. coli-derived recombinant human GLI1 Met1-Glu234 Accession # P08151.
Detects endogenous human and mouse GLI1.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 200 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About GLI / GLI1

P08151 NM_005269 NP_005260.1

GLI1 Antibody, Gli-1 Antibody, GLI family zinc finger 1 Antibody, Glioma-associated oncogene Antibody, Glioma-associated oncogene 1 Antibody, Oncogene GLI Antibody, GLI Antibody, Zinc finger protein GLI1 Antibody

Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.

Immunocytochemistry

Immunocytochemistry
GLI-1 in A1 72 Human Cell Line. GLI-1 was detected in immersion fixed A172 human glioblastoma cell line using 10 ug/ml Human/Mouse GLI-1 Monoclonal Antibody for 3 hours at room temperature. Cells were stained with the NorthernLights 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel).

Western blot

Western blot
Detection of Human/Mouse GLI-1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 ug/ml of Human/Mouse GLI-1 Monoclonal Antibody followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for GLI-1 at approximately 110 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/6/2016

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