Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
GLI1 Antibody, Gli-1 Antibody, GLI family zinc finger 1 Antibody, Glioma-associated oncogene Antibody, Glioma-associated oncogene 1 Antibody, Oncogene GLI Antibody, GLI Antibody, Zinc finger protein GLI1 Antibody
Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
GLI-1 in A1 72 Human Cell Line. GLI-1 was detected in immersion fixed A172 human glioblastoma cell line using 10 ug/ml Human/Mouse GLI-1 Monoclonal Antibody for 3 hours at room temperature. Cells were stained with the NorthernLights 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel).
Detection of Human/Mouse GLI-1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 ug/ml of Human/Mouse GLI-1 Monoclonal Antibody followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for GLI-1 at approximately 110 kD (as indicated). This experiment was conducted under reducing conditions.
Requested From: United States
Date Requested: 2/22/2017