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Anti-GLE1 Antibody (aa218-267) LS-C81875

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Alternate Forms of this Antibody

LS-C458775 FITC Conjugated
LS-C458776 Biotin Conjugated
LS-C458777 HRP Conjugated

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Rabbit Polyclonal (IgG) to Human GLE1
Human, Horse
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human GLE1
Rabbit
Human, Horse (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bovine, Dog (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

Western blot (applications tested for the base form of this product only)

Specificity and Use

GLE1 antibody was raised against synthetic peptide located between aa218-267 of human GLE1 (Q53GS7, NP_001490). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Marmoset, Panda, Horse (100%); Monkey, Mouse, Rat, Dog, Bovine, Opossum (92%); Pig, Platypus (85%); Turkey, Zebra finch, Chicken (84%).
LS-E19658 - Lyophilized - 100 µg - $145.00
aa218-267
Human GLE1 / GLE1L
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GLE1

Q53GS7 NM_001003722 NP_001003722.1

GLE1 Antibody, GLE1L Antibody, GLE1-like protein Antibody, LCCS1 Antibody, LCCS Antibody, GLE1-like, RNA export mediator Antibody, HGLE1 Antibody, Nucleoporin GLE1 Antibody

GLE1 is a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes.

Western blot

Western blot
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.
 

Requested From: United States
Date Requested: 8/30/2016

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