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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5511-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Rat GJB2 / CX26 / Connexin 26 Antibody (clone CX‑12H10, Cytoplasmic Domain, IHC, WB) LS‑B5511
IHC‑plus™ Monoclonal Mouse anti‑Rat GJB2 / CX26 / Connexin 26 Antibody (clone CX‑12H10, Cytoplasmic Domain, IHC, WB) LS‑B5511
Note: This antibody replaces LS-C14523
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
Connexin 26 antibody LS-B5511 is an unconjugated mouse monoclonal antibody to Connexin 26 (GJB2 / CX26) (Cytoplasmic Domain) from rat. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Rat GJB2 / CX26 / Connexin 26
Synonyms
GJB2 | Connexin 26 | Connexin-26 | DFNB1A | DFNA3 | DFNB1 | Gap junction protein beta 2 | KID | HID | PPK | NSRD1 | CX26 | DFNA3A
Host
Mouse
Reactivity
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
CX-12H10
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26. (4,5)
Epitope
Cytoplasmic Domain
Specificity
Reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5kD. Although the polyclonal anti-Cx26 antibody (feline. No. 71-0500) raised against the same sequence as this monoclonal anti-Cx26 antibody cross-reacts with Cx30, this monoclonal anti-Cx26 appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions. Rat, mouse and human. Reactivity of this antibody on Western blots has been confirmed by using extracts from mouse liver, mouse brain and rat brain.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Western blot (1 µg/ml)
- ELISA (0.1 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Immunohistochemistry (Frozen): 1-2 ug/ml. Western Blot: 1 ug/ml. Reactivity confirmed using extracts from mouse liver, mouse brain and rat brain.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GJB2 / CX26 / Connexin 26
Validation
Anti-GJB2 antibody IHC of rat intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-GJB2 antibody IHC of rat intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ GJB2 / CX26 / Connexin 26 Antibody (Cytoplasmic Domain, clone CX-12H10) for IHC, WB/Western, ELISA LS-B5511 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Mammary gland specific knockdown of the physiological surge in Cx26 during lactation retains normal mammary gland development and function. Stewart MK, Plante I, Bechberger JF, Naus CC, Laird DW. PloS one. 2014 9:e101546. (WB; Rat)
Customer Reviews (0)
Featured Products
Species:
Mouse, Human, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, Western blot, ELISA
Reactivity:
Human
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
