Mouse GJA5 / CX40 / Connexin 40

Rabbit

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

Synthetic peptide derived from an internal region of the mouse Connexin 40 (Cx40) protein.

Internal

Recognizes mouse Connexin 40 protein at ~40kD. Reactivity has been confirmed with mouse heart homogenates by Western blotting and mouse heart tissue by immunohistochemistry.

• IHC (1 - 3 µg/ml)
• Immunofluorescence (1 - 3 µg/ml)
• Western blot (1 - 3 µg/ml)

Suitable for use in Western Blot, Immunofluorescence, and Immunohistochemistry. Western Blot: 1-3 ug/ml. Immunofluorescence: 1-3 ug/ml. Immunohistochemistry: 1-3 ug/ml. NOTE: For IHC mice were perfused transcardially with 3 ml pre-fixative (50mm phosphate buffer, 0.1% sodium nitrate, 1u/ml heparin), followed by 40 ml fixative (0.16m sodium phosphate buffer, 0.2% picric acid, and 1-,2-, or 4% paraformaldehyde), and perfusion with 10 ml sucrose wash. Tissues were then stored in cryoprotectant for 24 hours.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P36382 NCBI: NM_005266 NP_005257.2