Mouse GJA5 / CX40 / Connexin 40

Rabbit

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the C-Terminus of the mouse and rat Connexin 40 (CX40) proteins. The human sequence at this location differs by only one amino acid.

C-Terminus

Reacts with the mouse Connexin 40 protein. On Western Blots, it identifies the target band at ~40kD. Reactivity has been confirmed with mouse heart homogenates by Western Blot, mouse heart and lung homogenates by immunoprecipitation, and mouse heart tissue by immunohistochemistry and immunofluorescence. Species cross-reactivity: Cross-reactivity with rat and human CX40 proteins is expected based on amino acid sequence homology.

• IHC
• IHC - Frozen
• Immunofluorescence (1 - 3 µg/ml)
• Western blot (1 - 3 µg/ml)
• Immunoprecipitation

Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Western Blot: 1-3 ug/ml. Immunofluorescence: 1-3 ug/ml. Immunoprecipitation: 7 ug/reaction. Immunohistochemistry (Frozen sections): 1-3 ug/ml; For IHC, mice were perfused transcardially with 3 ml pre-fixative (50 mM phosphate buffer, 0.1% sodium nitrite, 1 unit/ml heparin),. followed by 40 ml fixative (0.16M sodium phosphate buffer, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10 ml sucrose wash (10% sucrose in 25 mM sodium phosphate buffer, pH 7.4). Tissues were then stored in cryoprotectant (10% sucrose, 25 mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.

PBS, pH 7.4, 0.1% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P36382 NCBI: NM_005266 NP_005257.2