Mouse GJA3 / CX46 / Connexin 46

Rabbit

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

Synthetic peptide derived from an internal region of the mouse Connexin 46 protein.

Internal

Recognizes mouse Connexin 46 protein. Species sequence homology: rat, Chinese hamster and Golden Hamster.

• IHC
• IHC - Frozen
• Immunofluorescence (3 µg/ml)
• Western blot (1 - 3 µg/ml)

Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Immunohistochemistry (Frozen): 3 ug/ml. Western Blot: 1-3 ug/ml. Identifies a single band at ~46kD. Immunofluorescence: 3 ug/ml. Note: For IHC, mice were perfused transcardially with 3 ml pre-fixative (50 mM phosphate buffer, 0.1% sodium nitrite, 1 unit/ml heparin), followed by 40mL fixative (0.16M sodium phosphate buffer, pH 7.6, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10 ml sucrose wash (10% sucrose in 25 mM sodium phosphate buffer, pH 7.4. Tissues were then stored in cryoprotectant (10% sucrose, 25 mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9Y6H8 NCBI: NM_021954 NP_068773.2