Human GGA3

Rabbit

Human, Chimpanzee (tested or 100% immunogen sequence identity)

Chimpanzee (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 400-425 of Human GGA3.

A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity may occur with GGA3 from macaque (93% homology) and orangutan (87% homology) sources. The immunizing sequence is present on both the long (isoform 1) and short (isoform 2) transcript variants for this protein.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (1:500 - 1:3000)
• Immunoprecipitation (1:100)
• ELISA (1:10000 - 1:50000)

Immunohistochemistry: LS-B373 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B373 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9NZ52 NCBI: D63876 BAA09926.1