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Anti-GFP Antibody (TR) LS-C154206

Ordering

Wt. Vol. Conc. Price
1000 µg 1000 µl - $455
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular GFP Antibodies

Anti-GFP Antibody LS-C67081
Goat Polyclonal (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-GFP Antibody LS-C121913
Mouse Monoclonal (IgG1) to Aequorea victoria GFP
Aequorea victoria
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated
Anti-GFP Antibody (clone 9F9.F9) LS-C154208
Mouse Monoclonal [clone 9F9.F9] (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Aequorea victoria GFP
Aequorea victoria
Immunofluorescence, Western blot, ELISA
TR Conjugated

Details

Aequorea victoria GFP
Goat
Aequorea victoria (tested or 100% immunogen sequence identity)
IgG Polyclonal
Texas Red
Immunoaffinity purified
Unmodified

Applications

  • Immunofluorescence (1:500 - 1:2500)
  • Western blot (1:10000)
  • ELISA (1:20000)

Specificity and Use

GFP antibody was raised against recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% sodium azide
1,000 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About GFP

P42212 M62653 P42212

GFP Antibody

Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

Requested From: United States
Date Requested: 12/2/2016

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