Target
Aequorea victoria GFP
Reactivity
Aequorea victoria
(tested or 100% immunogen sequence identity)
Conjugations
Tetramethyl Rhodamine Iso-Thiocyanate
Purification
Affinity chromatography
Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Applications
- Immunofluorescence (1:500 - 1:2500)
- Western blot (1:10000)
- Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISA for direct binding of antigen recognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited to titrate GFP in solution using either form of the antibody as the capture or detection antibodies. The detection antibody is typically conjugated to biotin and complexed with streptavidin-HRP. Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Peroxidase conjugated anti-GFP assayed by immunoblot shows a 42 kD band when reacted with GFP on a western blot. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee