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Anti-GFP Antibody LS-C50830


Wt. Vol. Conc. Price
250 µg - - Unavailable

Most Popular GFP Antibodies

Anti-GFP Antibody (C-Terminus) LS-C51736
Rabbit Polyclonal to Aequorea victoria GFP
Aequorea victoria
Western blot, Immunoprecipitation
Anti-GFP Antibody LS-C121913
Mouse Monoclonal (IgG1) to Aequorea victoria GFP
Aequorea victoria
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Anti-GFP Antibody (clone 9F9.F9) LS-C154208
Mouse Monoclonal [clone 9F9.F9] (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC, Immunofluorescence, Western blot, ELISA
Western blot Image

100% Guaranteed 100% Guaranteed
Chicken Polyclonal (IgY) to Aequorea victoria GFP
Aequorea victoria
ICC, Western blot, Immunoprecipitation (applications tested for the base form of this product only)


Aequorea victoria GFP
Aequorea victoria (tested or 100% immunogen sequence identity)
IgY Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.


  • ICC
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)

Specificity and Use

GFP antibody was raised against affinity purified, recombinant green fluorescent protein (GFP) containing a 6-His tag (single band, SDS-PAGE).
Recognizes green fluorescent protein (GFP). Has been used to detect GFP in primary neurons and zebrafish fibroblasts.
Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Western Blot: Detects a band of the proper molecular weight, 30kD, in lysates from E. coli expressing GFP, but not in E. coli that do not express or in lysates prepared from E. coli containing the GFP-expressing plasmid but not induced, detect GFP in lysates from COS or primary neurons or fibroblast cells transfected with plasmids directing expression GFP or a tau-GFP fusion protein. Did not detect any signal when 50 ug of protein from a rat brain lysate (negative control) was fractionated on the SDS-PAGE. Titers were 1:5000-1:10000 when detected with an anti-chicken HRP conjugated secondary by ECL or by a colorimetric assay using DAB. Immunocytochemistry: GFP can be detected in cells that are transfected with a plasmid directing the expression of either GFP or a GFP-Fusion protein. Cells that do not express GFP exhibit no detectable staining. Binding was determined using HRP conjugated, anti-chicken secondary antibodies and visualized using DAB. The anti-GFP antibodies were used at dilutions of 1:1000-1:2500. Cells were fixed using 4% formaldehyde in PBS, pH 7.4. For immunocytochemistry, the cultures were fixed in 4% formaldehyde in PBS, pH 7.4, for 20 minutes at RT. The cells are permeabilized with 0.2% Triton X-100 and 2% serum (corresponding to the animal in which the secondary antibody was raised) in PBS for 20 minutes at RT. Cell cultures are then incubated with primary antisera in PBS containing 2% serum (as above), at 4?C, overnight. Has also been used successfully with the avidin/biotin/peroxidase detection system. Cultures were washed four times (10 minutes each) between steps, and reaction product is developed with a 0.05% diaminobenzidine/ 0.02% hydrogen peroxide solution on ice for 8-10 minutes. Has been used to detect GFP in primary neurons and zebrafish fibroblasts.


10 mM sodium chloride, 0.02% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GFP

P42212 M62653 P42212

GFP Antibody

Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

Requested From: 
Date Requested: 3/23/2017

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