Aequorea victoria GFP

Rabbit

Aequorea victoria (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

Highly purified recombinant GFP made in E. coli.

Recognizes Green Fluorescent Protein (GFP). Species cross-reactivity: all variants of Aequorea victoria GFP; S65T-GFP, RS-GFP, YFP and EGFP. Detects 5 ng of recombinant GFP (ECL) with 1 minute exposure or less.

• IHC
• IHC - Paraffin (1:2500 - 1:10000)
• ICC (1:200 - 1:1000)
• Immunofluorescence
• Western blot (1:2500 - 1:10000)
• Immunoprecipitation

Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry, Immunohistochemistry, Immunofluorescence and Immunogold Electron Microscopy. Western Blot: 1:2500-1:10000 in 1X BLOTTO or 3% BSA in PBS. Detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins. Immunocytochemistry: 1:200-1:1000 in 4% normal serum. Very good signal on recombinant YES-GFP chimeras expressed in COS cells. Immunohistochemistry: 1:200 with HRP detection systems. Has been shown to be useful on mouse sections fixed with formalin. Immunohistochemistry: 1:200 for paraffin sections. Requires heat mediated antigen retrieval via the microwave method before IHC staining (13min in 10 mM citrate buffer pH6 followed by rapid cooling on ice). Immunoprecipitation: 1 ul per 10cm tissue culture dish of transfected cells. Use 10 ul protein A agarose CL4B to precipitate immune complex. Immunogold Electron Microscopy: 1:100.

PBS, pH 7.2, 0.01% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P42212 NCBI: M62653 P42212