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Anti-GFP Antibody LS-C154219


Wt. Vol. Conc. Price
100 µg - 1 mg/ml $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular GFP Antibodies

Anti-GFP Antibody (clone N/K) LS-C58894
Mouse Monoclonal [clone N/K] (IgG1) to Aequorea victoria GFP
Aequorea victoria
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Anti-GFP Antibody LS-C121913
Mouse Monoclonal (IgG1) to Aequorea victoria GFP
Aequorea victoria
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Anti-GFP Antibody (clone 9F9.F9) LS-C154208
Mouse Monoclonal [clone 9F9.F9] (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC, Immunofluorescence, Western blot, ELISA
Western blot Image

100% Guaranteed 100% Guaranteed Publications Publications (5)
Rabbit Polyclonal (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC, Western blot, ELISA


Aequorea victoria GFP
Aequorea victoria (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC (1:200 - 1:3000)
  • Western blot (1:500 - 1:5000)

Specificity and Use

GFP antibody was raised against the immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Anti-GFP antibody is designed to detect GFP and its variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP, and EGFP. GFP antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (5)

Splice variants of the dual specificity tyrosine phosphorylation-regulated kinase 4 (DYRK4) differ in their subcellular localization and catalytic activity. Papadopoulos C, Arato K, Lilienthal E, Zerweck J, Schutkowski M, Chatain N, Mller-Newen G, Becker W, de la Luna S. The Journal of biological chemistry. 2011 286:5494-505. [PubMed:21127067] [PMC:PMC3037663]
The syntaxin 4 N terminus regulates its basolateral targeting by munc18c-dependent and -independent mechanisms. Torres J, Funk HM, Zegers MM, ter Beest MB. The Journal of biological chemistry. 2011 286:10834-46. [PubMed:21278252] [PMC:PMC3060534]
Vaccinia extracellular virions enter cells by macropinocytosis and acid-activated membrane rupture. Schmidt FI, Bleck CK, Helenius A, Mercer J. The EMBO journal. 2011 30:3647-61. [PubMed:21792173] [PMC:PMC3181475]
Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Rizzolio F, Lucchetti C, Caligiuri I, Marchesi I, Caputo M, Klein-Szanto AJ, Bagella L, Castronovo M, Giordano A. Cell death and differentiation. 2012 19:1152-61. [PubMed:22322860] [PMC:PMC3374078]
High-throughput, multiplexed analysis of 3-nitrotyrosine in individual proteins. Jin H, Zangar RC. Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]. 2012 Unit 17.15. [PubMed:22511115]

About GFP

P42212 M62653 P42212

GFP Antibody

Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

Western blot

Western blot
Western blot of Rabbit anti-GFP antibody. Lane 1: Wild type GFP (0.1 g) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 Goat-a-Rabbit IgG [H&L] MX10 (611-132-122) at 1:10000 for 45 min at RT. Block: 5% BLOTTO in PBS overnight at 4C. Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.

Western blot

Western blot
Anti-GFP Antibody - Western Blot. Western blot of GFP protein detected with polyclonal anti-GFP antibody. Wild type GFP (0.1 ug) was used to spike 30 ug of a HeLa whole cell lysate. This antibody detects a 27 kD band corresponding to the epitope tag GFP. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed overnight at 4C with the primary antibody diluted in 5% BLOTTO to 1:1000, followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Goat-a-Rabbit IgG [H&L] MX10 (. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 10/23/2016

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