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Anti-GFP Antibody LS-C154312

Ordering

Wt. Vol. Conc. Price
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GFP Antibodies

Anti-GFP Antibody LS-C67081
Goat Polyclonal (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-GFP Antibody LS-C121913
Mouse Monoclonal (IgG1) to Aequorea victoria GFP
Aequorea victoria
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated
Anti-GFP Antibody (clone 9F9.F9) LS-C154208
Mouse Monoclonal [clone 9F9.F9] (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Chicken Polyclonal (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Aequorea victoria GFP
Chicken
Aequorea victoria (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC (1:200 - 1:1000)
  • Immunofluorescence
  • Western blot (1:200 - 1:1000)
  • ELISA

Specificity and Use

GFP antibody was raised against the immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluor-escence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

1
The role of PSD-95 and cypin in morphological changes in dendrites following sublethal NMDA exposure. Tseng CY, Firestein BL. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:15468-80. [PubMed:22031893]

About GFP

P42212 M62653 P42212

GFP Antibody

Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

Western blot

Western blot
Anti-GFP Antibody - Western Blot. Western blot of GFP protein detected with polyclonal anti-GFP antibody. Lane 1 shows negative control staining of 20 ug of mouse spleen lysate. Lane 2 shows staining of mouse spleen lysate spiked with 50 ng of wt GFP. This antibody detects a 27 kD band corresponding to the GFP epitope tag commonly used in recombinant constructs. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE followed by transfer to nitrocellulose using standard methods. After blocking with 5% BSA in PBS, the membrane was probed for 2 h at room temperature with the primary antibody diluted in 5% BSA to 2 ug/ml, followed by washes and reaction with a 1:20000 dilution of IRDye800 Conjugated Affinity Purified anti-Chicken IgG [H&L] [Goat] MX10 (. The IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/4/2016

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