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Anti-GFP Antibody (clone 9F9.F9) LS-C154208

Catalog Size Price
LS-C154208-1000 1000 µg (1 mg/ml) Unavailable

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Western blot Image

100% Guaranteed Publications (4)
Mouse Monoclonal [clone 9F9.F9] (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC - Frozen, Immunofluorescence, Western blot, ELISA


Aequorea victoria GFP
Aequorea victoria (tested or 100% immunogen sequence identity)
IgG Monoclonal [9F9.F9]
Protein A purified


  • IHC - Frozen (1:1000 - 1:5000)
  • Immunofluorescence
  • Western blot (1:3000 - 1:30000)

Specificity and Use

GFP antibody was raised against recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with a polyclonal anti-GFP antibody as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. Tested for cross reactivity with RFP only in ELISA.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.
For research use only.

Publications (4)

ORF73-null murine gammaherpesvirus 68 reveals roles for mLANA and p53 in virus replication. Forrest JC, Paden CR, Allen RD, Collins J, Speck SH. Journal of virology. 2007 81:11957-71. [PubMed:17699571] [PMC:PMC2168792]
Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus. Yang F, Camp DG, Gritsenko MA, Luo Q, Kelly RT, Clauss TR, Brinkley WR, Smith RD, Stenoien DL. Journal of cell science. 2007 120:4060-70. [PubMed:17971412]
Type A GABA-receptor-dependent synaptic transmission sculpts dendritic arbor structure in Xenopus tadpoles in vivo. Shen W, Da Silva JS, He H, Cline HT. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2009 29:5032-43. [PubMed:19369572] [PMC:PMC2706946]
Lef-1 isoforms regulate different target genes and reduce cellular adhesion. Jesse S, Koenig A, Ellenrieder V, Menke A. International journal of cancer. Journal international du cancer. 2010 126:1109-20. [PubMed:19653274]

About GFP

P42212 M62653 P42212

GFP Antibody

Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

Western blot

Western blot
Anti-GFP Chemiluminescent Kit for Western blot. Known amounts of recombinant GFP protein ( were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4°C prior to probing the blot with the anti-GFP monoclonal antibody LS-C154208 diluted 1:1000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n LS-C60772) was performed at a dilution of 1:20000 for 1h at 4°C. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).

Western blot

Western blot
Anti-GFP Antibody - Western Blot. Western blot of GFP recombinant protein detected with monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. Mab anti-GFP detects a 27 kD band corresponding to the epitope tag GFP. The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1.0 mg/ml for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] MX10 (. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: 
Date Requested: 3/18/2018

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