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Anti-GFP Antibody (clone 9F9.F9, AP) LS-C154211

Ordering

Wt. Vol. Conc. Price
1000 µg - - $785
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GFP Antibodies

Anti-GFP Antibody (C-Terminus) LS-C51736
Rabbit Polyclonal to Aequorea victoria GFP
Aequorea victoria
Western blot, Immunoprecipitation
Unconjugated
Anti-GFP Antibody (clone 9F9.F9) LS-C154208
Mouse Monoclonal [clone 9F9.F9] (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot Image
Anti-GFP Antibody LS-C121913
Mouse Monoclonal (IgG1) to Aequorea victoria GFP
Aequorea victoria
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 9F9.F9] (IgG) to Aequorea victoria GFP
Aequorea victoria
IHC, Western blot, ELISA
AP Conjugated

Details

Aequorea victoria GFP
Mouse
Aequorea victoria (tested or 100% immunogen sequence identity)
IgG Monoclonal [9F9.F9]
Alkaline Phosphatase
Protein A purified
Unmodified

Applications

  • IHC (1:200 - 1:1000)
  • Western blot (1:500 - 1:2500)
  • ELISA

Specificity and Use

GFP antibody was raised against the immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with a polyclonal anti-GFP antibody as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.05 M Tris chloride, 0.15 M sodium chloride, 1 mM magnesiuim chloride, 0.1 mM zinc chloride, pH 8.0, 50% glycerol, 1% BSA, 0.01% sodium azide, sterile filtered
Store at 4°C. Do not freeze.
For research use only.

About GFP

P42212 M62653 P42212

GFP Antibody

Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

Western blot

Western blot
GFP Antibody Alkaline Phosphatase Conjugated Western Blot. Western Blot of Mouse anti-GFP Antibody Alkaline Phosphatase Conjugated Lane 1: GFP Load: 100 ng per lane Secondary antibody: GFP Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 28 kD, 28 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/7/2016

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