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| Catalog Number | Size | Price |
|---|---|---|
| LS-C154288-100 | 100 µg (1 mg/ml) | $544 |
Polyclonal Rabbit anti‑Aequorea victoria GFP Antibody (Biotin, IHC, IF, WB) LS‑C154288
Polyclonal Rabbit anti‑Aequorea victoria GFP Antibody (Biotin, IHC, IF, WB) LS‑C154288
Antibody:
GFP Rabbit anti-Aequorea victoria Polyclonal (Biotin) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Biotin, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
GFP Rabbit anti-Aequorea victoria Polyclonal (Biotin) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Biotin, Unmodified
Specifications
Description
GFP antibody LS-C154288 is a biotin-conjugated rabbit polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IF, IHC and WB. Cited in 1 publication.
Host
Rabbit
Reactivity
Aequorea victoria
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse and Rat Serum Proteins.
Applications
- IHC (1:1000 - 1:5000)
- Immunofluorescence (1:5000)
- Western blot (1:2000 - 1:10000)
- ELISA (1:10000 - 1:50000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
LSBio Ratings
GFP Antibody (Biotin) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-C154288 has an LSBio Rating of
Publications (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays. Zangar RC, Daly DS, White AM, Servoss SL, Tan RM, Collett JR. Journal of proteome research. 2009 8:3937-43.
Customer Reviews (0)
Featured Products
Species:
Aequorea victoria
Applications:
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Aequorea victoria
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Species:
Aequorea victoria
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Aequorea victoria
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Species:
Aequorea victoria
Applications:
IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/22/2024
Date Requested: 4/22/2024
