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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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GFP Antibody (AP) LS‑C154196
GFP antibody LS-C154196 is an AP-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C154196-1000
1000 µg
Unavailable

Popular GFP Products

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Species: Aequorea victoria
Applications: Western blot, Immunoprecipitation
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Species: Aequorea victoria
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Antibody
Species: Aequorea victoria
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Aequorea victoria
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Anti-GFP Chemiluminescent Kit for Western blot. Known amounts of recombinant GFP protein ( were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4°C prior to probing the blot with the anti-GFP monoclonal antibody LS-C154208 diluted 1:1000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n LS-C60772) was performed at a dilution of 1:20000 for 1h at 4°C. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
Species: Aequorea victoria
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, ELISA

Product Description

GFP antibody LS-C154196 is an AP-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
About GFP
Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. P42212 M62653 P42212

GFP Antibody


Specifications

Target
Aequorea victoria GFP
Host
Goat
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Alkaline Phosphatase
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:500 - 1:2500)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GFP antibody was raised against recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Usage
Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.05 M Tris chloride, 0.15 M sodium chloride, 1 mM magnesiuim chloride, 0.1 mM zinc chloride, pH 8.0, 50% glycerol, 1% BSA, 0.1% sodium azide, sterile filtered
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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