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Anti-GFAP Antibody (N-Terminus) LS-C359007

Catalog Size Price
LS-C359007-30 30 µl (1 mg/ml) Unavailable
LS-C359007-100 100 µl (1 mg/ml) Unavailable
LS-C359007-200 200 µl (1 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human GFAP
Human, Mouse, Bovine
IHC - Paraffin, ICC, Immunofluorescence, Western blot


Human GFAP
Human, Mouse, Bovine (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)

Specificity and Use

GFAP antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human GFAP.
Recognizes endogenous levels of GFAP (pS38) protein.


PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
Aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
For research use only.

About GFAP

P14136 NM_002055 NP_002046.1

GFAP Antibody

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.


Immunohistochemical analysis of GFAP (pS38) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.


Immunofluorescent analysis of GFAP (pS38) staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot
Western blot analysis of GFAP (pS38) expression in COLO205 (A); mouse brain (B) whole cell lysates.

Requested From: 
Date Requested: 3/24/2018

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