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Catalog Number Size Price
LS-C330862-20 20 µl $275 
LS-C330862-50 50 µl $311 
LS-C330862-100 100 µl $389 
LS-C330862-200 200 µl $503 
GFAP Antibody - Immunohistochemistry of paraffin-embedded rat brain using GFAP antibodyat dilution of 1:75 (40x lens).
GFAP Antibody - Immunohistochemistry of frozen neuroglial cells.
GFAP Antibody - Immunofluorescence analysis of U-251MG cells using GFAP antibodyat dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
GFAP Antibody - Western blot analysis of extracts of various cell lines, using GFAP antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
GFAP Antibody - Immunohistochemistry of paraffin-embedded rat brain using GFAP antibodyat dilution of 1:75 (40x lens).
GFAP Antibody - Immunohistochemistry of frozen neuroglial cells.
GFAP Antibody - Immunofluorescence analysis of U-251MG cells using GFAP antibodyat dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
GFAP Antibody - Western blot analysis of extracts of various cell lines, using GFAP antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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Polyclonal Rabbit anti‑Human GFAP Antibody (IHC, IF, WB) LS‑C330862

Polyclonal Rabbit anti‑Human GFAP Antibody (IHC, IF, WB) LS‑C330862

Antibody:
GFAP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C330862-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GFAP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
GFAP antibody LS-C330862 is an unconjugated rabbit polyclonal antibody to GFAP from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human GFAP
Synonyms
GFAP
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-Terminus of human GFAP (NP_002046.1). RGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM
Specificity
Human GFAP
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 49kDa/50kDa, while the observed MW by Western blot was 50kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFAP
P14136 NM_002055 NP_002046.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/15/2024