Human GFAP

Chicken

Human (tested or 100% immunogen sequence identity)

IgY Polyclonal

Unconjugated

Purified

Unmodified

The initial challenge was performed with a preparation of recombinant GFAP expressed in bacteria and highly purified. Subsequent boosts were performed with GFAP purified from a Triton X-100 extract of myelin associated material from bovine spinal cord, following an axonal flotation procedure. The GFAP was further purified by centrifugation and ion exchange chromatography in 6m urea on DEAE cellulose.

Human GFAP

• IHC (1:1000)
• ICC (1:1000)
• Western blot (1:100000)

For immunocytochemistry on cells in tissue culture or in tissue sections, try this antibody at about between about 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme linked methods. For immunoblotting 1:50000 is recommended. Expect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band.

15mM sodium azide

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14136 NCBI: NM_002055 NP_002046.1