Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
GFAP antibody was raised against glial Fibrillary Acidic Protein (51-52kD). Cellular Localization: Cytoplasmic.
Stains astrocytes, glial cells, ependymal cells and their corresponding tumors. Does not stain many types of neural tumors such as neuroblastomas, Schwannomas and extra-CNS tumors. Species cross-reactivity: human, porcine, rat, chicken.
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Western Blot: 1-2 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1-2 ug/ml for 30 min at RT. Note: No special pretreatment required prior to staining of formalin-fixed, paraffin-embedded sections. Positive control: IMR-5 cells, Brain or astrocytoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, pH 7.4
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.