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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GFAP Antibody (aa417‑432) LS‑C357892
GFAP antibody LS-C357892 is an unconjugated rabbit polyclonal antibody to GFAP from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C357892-100
100 µg (0.5 mg/ml)
$415

Popular GFAP Products

Anti-GFAP antibody IHC of human brain, cortex, astrocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2192 concentration 5 ug/ml.
Species: Human, Monkey, Rat, Bat, Bovine, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-GFAP antibody IHC of human cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4775 dilution 1:100.
Species: Bovine, Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
Anti-GFAP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B285 dilution 1:500.
Species: Pig, Human, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin
Anti-GFAP antibody IHC of human brain, astrocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6299 dilution 1:100.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Anti-GFAP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5176 dilution 1:50.
Species: Bovine, Human, Monkey, Mouse, Rat, Guinea pig, Hamster, Sheep, Chicken
Applications: IHC, IHC - Paraffin

Product Description

GFAP antibody LS-C357892 is an unconjugated rabbit polyclonal antibody to GFAP from human, mouse, rat and other species. Validated for IHC and WB.
About GFAP
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. P14136 NM_002055 NP_002046.1

GFAP Antibody


Specifications

Target
Human GFAP
Host
Rabbit
Reactivity
Human, Gibbon, Monkey, Mouse, Rat, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Dog, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GFAP antibody was raised against a synthetic peptide corresponding to a sequence at the C-terminus of human GFAP (417-432aa DGEVIKESKQEHKDVM). Percent identity by BLAST analysis: Human, Gibbon, Galago, Marmoset, Rat, Cat, Rabbit, Horse (100%); Pig, Dog (94%); Mouse (88%); Opossum (81%).
Epitope
aa417-432
Specificity
Human GFAP
Usage
Glial fibrillary acidic protein is an intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. Expression is also seen in rat kidney glomeruli and peritubular fibroblasts, leydig cells of testis, skin keratinocytes, osteocytes of bones, chondrocytes of epiglottis, bronchus, and stellate-shaped cells of the pancreas and liver. Its expression is essential for normal white matter architecture and blood-brain barrier integrity, and its absence leads to late-onset CNS dysmyelination. The protein has also been shown to play a role in mitosis by adjusting the filament network present in the cell. During mitosis, there is an increase in the amount of phosphorylated GFAP, and a movement of this modified protein to the cleavage furrow.
Presentation
Lyophilized from PBS, 2.5% BSA, 0.025% sodium azide/thimerosal.
Reconstitution
0.2 ml Sterile distilled water
Storage
Store at 4°C for 1 month or -20°C for longer. Avoid freeze/thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

IHC-P: GFAP antibody testing of rat brain tissue
IHC-P: GFAP antibody testing of rat brain tissue

Western blot

Western blot testing of GFAP antibody and Lane 1. rat brain, 2: mouse brain tissue lysate
Western blot testing of GFAP antibody and Lane 1. rat brain, 2: mouse brain tissue lysate

Western blot

Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain
Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain

Immunohistochemistry

IHC-P: GFAP antibody testing of rat brain tissue
IHC-P: GFAP antibody testing of rat brain tissue

Western blot

Western blot testing of GFAP antibody and Lane 1. rat brain, 2: mouse brain tissue lysate
Western blot testing of GFAP antibody and Lane 1. rat brain, 2: mouse brain tissue lysate

Western blot

Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain
Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain

Immunohistochemistry

IHC-P: GFAP antibody testing of rat brain tissue
IHC-P: GFAP antibody testing of rat brain tissue

Western blot

Western blot testing of GFAP antibody and Lane 1. rat brain, 2: mouse brain tissue lysate
Western blot testing of GFAP antibody and Lane 1. rat brain, 2: mouse brain tissue lysate

Western blot

Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain
Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain

Immunohistochemistry

IHC-P: GFAP antibody testing of rat brain tissue
IHC-P: GFAP antibody testing of rat brain tissue

Western blot

Western blot testing of GFAP antibody and Lane 1. rat brain, 2: mouse brain tissue lysate
Western blot testing of GFAP antibody and Lane 1. rat brain, 2: mouse brain tissue lysate

Western blot

Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain
Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain

Requested From: United States
Date Requested: 9/25/2018

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