Human, Gibbon, Monkey, Mouse, Rat, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Dog, Pig (at least 90% immunogen sequence identity)
- IHC - Paraffin (0.5 - 1 µg/ml)
- Western blot (0.5 - 1 µg/ml)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
GFAP antibody was raised against a synthetic peptide corresponding to a sequence at the C-terminus of human GFAP (417-432aa DGEVIKESKQEHKDVM). Percent identity by BLAST analysis: Human, Gibbon, Galago, Marmoset, Rat, Cat, Rabbit, Horse (100%); Pig, Dog (94%); Mouse (88%); Opossum (81%).
Glial fibrillary acidic protein is an intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. Expression is also seen in rat kidney glomeruli and peritubular fibroblasts, leydig cells of testis, skin keratinocytes, osteocytes of bones, chondrocytes of epiglottis, bronchus, and stellate-shaped cells of the pancreas and liver. Its expression is essential for normal white matter architecture and blood-brain barrier integrity, and its absence leads to late-onset CNS dysmyelination. The protein has also been shown to play a role in mitosis by adjusting the filament network present in the cell. During mitosis, there is an increase in the amount of phosphorylated GFAP, and a movement of this modified protein to the cleavage furrow.
Lyophilized from PBS, 2.5% BSA, 0.025% sodium azide/thimerosal.
0.2 ml Sterile distilled water
Store at 4°C for 1 month or -20°C for longer. Avoid freeze/thaw cycles.
For research use only.