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Anti-GDF3 Antibody LS-C104372

Note: This antibody replaces LS-C36581

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GDF3 Antibodies

Anti-GDF3 Antibody (aa35-53) IHC-plus™ LS-B807
Rabbit Polyclonal to Human GDF3
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GDF3 Antibody (aa29-59, APC) LS-C277774
Rabbit Polyclonal (IgG) to Human GDF3
Human, Mouse
IHC, Western blot, ELISA
APC Conjugated
Anti-GDF3 Antibody (aa29-59, PE) LS-C277777
Rabbit Polyclonal (IgG) to Human GDF3
Human, Mouse
IHC, Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human GDF3
Human
IHC, Western blot, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human GDF3
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

GDF3 antibody was raised against e.coli derived recombinant Human GDF-3.
Human GDF-3
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hGDF-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GDF-3 (LS-C104295) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGDF-3. Western Blot: To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDF-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About GDF3

Q9NR23 NM_020634 NP_065685.1

GDF3 Antibody, KFS3 Antibody, MCOPCB6 Antibody, GDF-3 Antibody, MCOP7 Antibody

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues.

Immunohistochemistry

Immunohistochemistry
IHC of GDF3 antibody LS-C104372. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of GDF3 antibody LS-C104372. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of GDF3 antibody LS-C104372. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of GDF3 antibody LS-C104372. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/2/2016

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