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Anti-GDF15 Antibody (N-Terminus) LS-C154241

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GDF15 Antibodies

Anti-GDF15 Antibody (aa286-299) IHC-plus™ LS-B2413
Goat Polyclonal to Human GDF15
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GDF15 Antibody (clone ME-6D10) LS-C89472
Mouse Monoclonal [clone ME-6D10] (IgG1) to Human GDF15
Human
Flow Cytometry, ELISA
Unconjugated
Anti-GDF15 Antibody (clone 6D12.H10.E4) IHC-plus™ LS-B8327
Rat Monoclonal [clone 6D12.H10.E4] (IgG) to Human GDF15
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human GDF15
Human
IHC, Western blot, ELISA
Unconjugated

Details

Human GDF15
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot (1:2000 - 1:100000)
  • ELISA

Specificity and Use

GDF15 antibody was raised against this affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
N-Terminus
This antibody specifically reacts with the amino terminal end of human NAG-1 protein from human tissues. A BLAST analysis was used to suggest partial reactivity with NAG-1 from chimpanzee and macaque based on a 92% homology. Multimeric forms of NAG-1 may be detected. Cross-reactivity with NAG-1 from other sources has not been determined.
This affinity purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 C-terminal specific antibodies. This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user. Expect bands in western blots of approximately 14 kD in size corresponding to NAG-1 monomer using the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About GDF15

Q99988 NM_004864 NP_004855.2

GDF15 Antibody, MIC1 Antibody, MIC-1 Antibody, NAG-1 Antibody, NRG-1 Antibody, NSAID-regulated gene 1 protein Antibody, PLAB Antibody, NSAID-activated gene 1 protein Antibody, Placental TGF-beta Antibody, PTGFB Antibody, GDF-15 Antibody, PTGF-beta Antibody

Growth differentiation factor 15 (GDF15) is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB. GDF15 mRNA is most abundant in the liver, with lower levels seen in some other tissues.

Western blot

Western blot
Anti-NAG-1/GDF15 (N-terminal specific) Antibody - Western Blot. Western blot of affinity purified anti-NAG-1/GDF15 (N-terminal specific) antibody shows detection of a 14 kD band corresponding to recombinant human NAG-1 purified from CHO cells. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is greatly diminished when this antibody is preincubated with the immunizing peptide prior to Western blotting.
 

Requested From: United States
Date Requested: 12/3/2016

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