Human GDF15

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to mature human NAG-1 protein.  Cross reactivity to MBP was removed via cross-adsorption chromatography.

This antibody reacts with endogenous NAG-1 protein from human tissues. A BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee based on 100% and 98% homology, respectively, and with macaque based on an 94% homology, and with mouse and rat based on 70% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.

• Western blot (1:800 - 1:1500)
• ELISA (1:500)

This Protein-A purified, MBP-cross-adsorbed antibody is suitable for ELISA and western blotting of human and mouse NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 C-terminal specific antibodies. This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user. Expect bands in western blots of approximately 14 kD in size corresponding to NAG-1 monomer using the appropriate cell lysate or extract.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q99988 NCBI: NM_004864 NP_004855.2