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Anti-GDF15 Antibody (C-Terminus) LS-C154321

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GDF15 Antibodies

Anti-GDF15 Antibody (aa286-299) IHC-plus™ LS-B2413
Goat Polyclonal to Human GDF15
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GDF15 Antibody (clone ME-6D10) LS-C89472
Mouse Monoclonal [clone ME-6D10] (IgG1) to Human GDF15
Human
Flow Cytometry, ELISA
Unconjugated
Anti-GDF15 Antibody (clone 6D12.H10.E4) IHC-plus™ LS-B8327
Rat Monoclonal [clone 6D12.H10.E4] (IgG) to Human GDF15
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse GDF15
Mouse, Human
Western blot, ELISA
Unconjugated

Details

Mouse GDF15
Rabbit
Mouse, Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:3000 - 1:7000)
  • ELISA

Specificity and Use

GDF15 antibody was raised against this affinity purified antibody was prepared by repeated immunizations with a peptide corresponding to an amino acid sequence near the C-terminal of mouse NAG-1 protein.
C-Terminus
This antibody reacts with endogenous NAG-1 protein from mouse and human tissues. A BLAST analysis suggests reactivity with NAG-1 from rat based on 100% homology. Partial reactivity is expected against swine, bovine and dog based on 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.
This affinity purified antibody is suitable for ELISA and western blotting of mouse and human NAG-1 protein. For detection of NAG-1 in mouse serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal) specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of approximately 14 and 28 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About GDF15

Q99988 NM_004864 NP_004855.2

GDF15 Antibody, MIC1 Antibody, MIC-1 Antibody, NAG-1 Antibody, NRG-1 Antibody, NSAID-regulated gene 1 protein Antibody, PLAB Antibody, NSAID-activated gene 1 protein Antibody, Placental TGF-beta Antibody, PTGFB Antibody, GDF-15 Antibody, PTGF-beta Antibody

Growth differentiation factor 15 (GDF15) is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB. GDF15 mRNA is most abundant in the liver, with lower levels seen in some other tissues.

Western blot

Western blot
Anti-Mouse NAG-1/GDF15 Antibody - Western Blot. Western blot of affinity purified anti-mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kD) purified from E. coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kD) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kD) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4C. Nag-1 was detected using a 1:40000 dilution of peroxidase conjugated Gt-a-Rabbit antibody (LS-C60865) in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/8/2016

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