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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2328-50 | 50 µl (1 mg/ml) | $485 |
IHC‑plus™ Polyclonal Rabbit anti‑Rat GCLC Antibody (aa295‑313, IHC, WB) LS‑B2328
IHC‑plus™ Polyclonal Rabbit anti‑Rat GCLC Antibody (aa295‑313, IHC, WB) LS‑B2328
Note: This antibody replaces LS-C15310
Antibody:
GCLC Rabbit anti-Rat Polyclonal (aa295-313) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Rat, Human, Monkey, Mouse, Bovine
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
GCLC Rabbit anti-Rat Polyclonal (aa295-313) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Rat, Human, Monkey, Mouse, Bovine
Format:
Unconjugated, Unmodified
Specifications
Description
GCLC antibody LS-B2328 is an unconjugated rabbit polyclonal antibody to GCLC (aa295-313) from rat. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
Target
Rat GCLC
Synonyms
GCLC | GCL | GCS heavy chain | Gamma-ECS | GCS | GLCL | GLCLC
Host
Rabbit
Reactivity
Rat, Human, Monkey, Mouse, Bovine
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
A synthetic peptide derived from corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS).
Epitope
aa295-313
Specificity
Recognizes the heavy subunit of rat GCS. Species cross-reactivity: human, monkey and mouse. Cross-reacts weakly with bovine.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
- Immunoprecipitation
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2328 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2328 was determined to be 5 ug/ml. Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 2.5-5 ug/ml for 2hrs at RT. Immunoprecipitation: 10 ug/mg protein lysate. Denatured verified. Use Protein A. Positive control: Jurkat, MAD109, PC12 cells.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GCLC
Validation
Anti-GCLC antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GCLC antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ GCLC Antibody (aa295-313) for IHC, WB/Western, IP, ELISA LS-B2328 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
