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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GCK / Germinal Center Kinase Antibody (aa288‑319) LS‑C99003
Germinal Center Kinase antibody LS-C99003 is an unconjugated rabbit polyclonal antibody to Germinal Center Kinase (GCK) from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C99003-400
400 µl
Unavailable

Popular GCK / Germinal Center Kinase Products

Antibody
Species: Human, Monkey, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Pig, Zebrafish
Applications: Western blot
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Immunohistochemistry of Human liver cancer using GCK Polyclonal Antibody at dilution of 1:40.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, ELISA

Product Description

Germinal Center Kinase antibody LS-C99003 is an unconjugated rabbit polyclonal antibody to Germinal Center Kinase (GCK) from human and mouse. Validated for IHC and WB.
About GCK / Germinal Center Kinase
Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Q12851 NM_004579 NP_004570.2

MAP4K2 Antibody, BL44 Antibody, GC kinase Antibody, Rab8 interacting protein Antibody, RAB8IP Antibody, Rab8-interacting protein Antibody, Germinal center kinase Antibody, MEK kinase kinase 2 Antibody, MEKKK 2 Antibody


Specifications

Target
Human GCK / Germinal Center Kinase
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8653 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99003. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa288-319
Specificity
This Germinal Center Kinase (GCK/MAP4K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-319 amino acids from the Central region of human Germinal Center Kinase (GCK/MAP4K2).
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

The anti-MAP4K2 antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.
The anti-MAP4K2 antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

The anti-MAP4K2 antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.
The anti-MAP4K2 antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

The anti-MAP4K2 antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.
The anti-MAP4K2 antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

The anti-MAP4K2 antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.
The anti-MAP4K2 antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.


Requested From: 
Date Requested: 6/18/2018

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