Human GCC2

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-QKLTNKNNKIED, from the internal region of the protein sequence according to NP_055450.1NP_852118.1.

aa1174-1185

Human GCC2. This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).

• IHC
• IHC - Paraffin (3.75 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:16000)

Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave an approx 60kD band in Human Lymph Node and Testis lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185kD according to NP_055450.1 and of 196kD according to NP_852118.1. The 60kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q8IWJ2 NCBI: NM_014635 NP_055450.1