(tested or 100% immunogen sequence identity)
Monkey, Rat, Bat, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
- IHC - Paraffin (3.75 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
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GCC2 antibody was raised against synthetic peptide C-QKLTNKNNKIED from an internal region of human GCC2 (NP_055450.1; NP_852118.1). Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Monkey, Marmoset, Rat, Hamster, Elephant, Bat, Horse, Rabbit (92%); Mouse, Bovine, Pig (83%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4938. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human GCC2. This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).
Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave an approx 60kD band in Human Lymph Node and Testis lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185kD according to NP_055450.1 and of 196kD according to NP_852118.1. The 60kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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