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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GCC2 Antibody (aa1174‑1185) IHC‑plus™ LS‑B4938 Best Seller
Note: This antibody replaces LS-C87221
GCC2 antibody LS-B4938 is an unconjugated goat polyclonal antibody to human GCC2. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4938-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

GCC2 antibody LS-B4938 is an unconjugated goat polyclonal antibody to human GCC2. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
About GCC2
Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Q8IWJ2 NM_014635 NP_055450.1

GCC2 Antibody, CLL-associated antigen KW-11 Antibody, CTCL tumor antigen se1-1 Antibody, GCC185 Antibody, GCC protein, 185-kD Antibody, Ran-binding protein 2-like 4 Antibody, RANBP2L4 Antibody, REN53 Antibody, KIAA0336 Antibody


Specifications

Target
Human GCC2
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rat, Bat, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GCC2 antibody was raised against synthetic peptide C-QKLTNKNNKIED from an internal region of human GCC2 (NP_055450.1; NP_852118.1). Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Monkey, Marmoset, Rat, Hamster, Elephant, Bat, Horse, Rabbit (92%); Mouse, Bovine, Pig (83%).
Blocking Peptide
LS-E26442 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4938. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1174-1185
Specificity
Human GCC2. This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave an approx 60kD band in Human Lymph Node and Testis lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185kD according to NP_055450.1 and of 196kD according to NP_852118.1. The 60kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-GCC2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-GCC2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.
Anti-GCC2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4938 concentration 3.75 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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