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GC / Vitamin D‑Binding Protein Antibody IHC‑plus™ LS‑B3318
Note: This antibody replaces LS-C18514
Vitamin D-Binding Protein antibody LS-B3318 is an unconjugated mouse monoclonal antibody to human Vitamin D-Binding Protein (GC). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
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Review This ProductPopular GC / Vitamin D-Binding Protein Products
Species:
Mouse
Applications:
Western blot, ELISA
Species:
Human
Applications:
ELISA
Product Description
Vitamin D-Binding Protein antibody LS-B3318 is an unconjugated mouse monoclonal antibody to human Vitamin D-Binding Protein (GC). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About GC / Vitamin D-Binding Protein
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
P02774
NM_000583
NP_000574.2
GC Antibody, Gc-globulin Antibody, HDBP Antibody, Group-specific component Antibody, GRD3 Antibody, VDB Antibody, VDBG Antibody, VDBP Antibody, Vitamin D-binding protein Antibody
Specifications
Target
Human GC / Vitamin D-Binding Protein
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
- ELISA (1:10000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Immunogen
Gc-globulin isolated from human plasma.
Specificity
Recognizes human Gc-globulin. Epitope specificity differs from those of clones 6F109, 6F110, and 6F111. Strong reaction is seen in ELISA with Gc-globulin coated directly onto the microtiter well and when tested in sandwich ELISA in combination with a polyclonal antibody against Gc-globulin. In Western blotting after SDS-PAGE reacts with Gc-globulin in both reduced as well as unreduced forms.
Usage
Immunohistochemistry: LS-B3318 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3318 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 15 mM sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Immunohistochemistry
Anti-GC / VDBP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3318 concentration 10 ug/ml.
Immunohistochemistry
Anti-GC / VDBP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3318 concentration 10 ug/ml.
Immunohistochemistry
Anti-GC / VDBP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3318 concentration 10 ug/ml.
Immunohistochemistry
Anti-GC / VDBP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3318 concentration 10 ug/ml.
Requested From:
Date Requested: 6/23/2018
Date Requested: 6/23/2018
