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Anti-GC / Vitamin D-Binding Protein Antibody IHC-plus™ LS-B3318
Note: This antibody replaces LS-C18514Most Popular GC / Vitamin D-Binding Protein Antibodies
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Details
| Human GC / Vitamin D-Binding Protein | |
| Mouse | |
| Human (tested or 100% immunogen sequence identity) | |
| IgG1,k Monoclonal | |
| Unconjugated | |
| Protein A/G purified | |
| Unmodified |
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
- ELISA (1:10000)
Specificity and Use
| Gc-globulin isolated from human plasma. | |
| Recognizes human Gc-globulin. Epitope specificity differs from those of clones 6F109, 6F110, and 6F111. Strong reaction is seen in ELISA with Gc-globulin coated directly onto the microtiter well and when tested in sandwich ELISA in combination with a polyclonal antibody against Gc-globulin. In Western blotting after SDS-PAGE reacts with Gc-globulin in both reduced as well as unreduced forms. | |
| Immunohistochemistry: LS-B3318 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3318 was determined to be 10 ug/ml. |
Packaging
| PBS, pH 7.4, 15 mM sodium azide. | |
| Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles. | |
| For research use only. |
About GC / Vitamin D-Binding Protein
P02774
NM_000583
NP_000574.2
GC Antibody, Gc-globulin Antibody, HDBP Antibody, Group-specific component Antibody, GRD3 Antibody, VDB Antibody, VDBG Antibody, VDBP Antibody, Vitamin D-binding protein Antibody
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
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Requested From:
Date Requested: 4/22/2018
Date Requested: 4/22/2018
