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Anti-GBE1 Antibody (clone 1D11) LS-C114902

Ordering

Wt. Vol. Conc. Price
- 100 µl - $350
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GBE1 Antibodies

Anti-GBE1 Antibody (aa527-556, Biotin) LS-C222598
Rabbit Polyclonal (IgG) to Human GBE1
Human, Mouse
ICC, Immunofluorescence, Western blot, ELISA
Biotin Conjugated
Anti-GBE1 Antibody (aa527-556, FITC) LS-C222599
Rabbit Polyclonal (IgG) to Human GBE1
Human, Mouse
ICC, Immunofluorescence, Western blot, ELISA
FITC Conjugated
Anti-GBE1 Antibody (aa527-556, PE) LS-C222601
Rabbit Polyclonal (IgG) to Human GBE1
Human, Mouse
ICC, Immunofluorescence, Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1D11] (IgG1) to Human GBE1
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human GBE1
Mouse
IgG1 Monoclonal [1D11]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

GBE1 antibody was raised against full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell
Human GBE1
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About GBE1

Q04446 NM_000158 NP_000149.3

GBE1 Antibody, APBD Antibody, Brancher enzyme Antibody, GBE Antibody, Glycogen-branching enzyme Antibody, GSD4 Antibody, Glycogen branching enzyme Antibody

GBE1 is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle.

Immunohistochemistry

Immunohistochemistry
Immunohistochemical staining of paraffin-embedded colon tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Immunofluorescence
Anti-GBE1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1.

Immunofluorescence

Immunofluorescence
Immunofluorescent staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody.

Western blot

Western blot
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GBE1 monoclonal antibody.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.

Flow Cytometry

Flow Cytometry
Flow cytometric analysis of Jurkat cells, using anti-GBE1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY GBE1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-GBE1 mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.
 

Requested From: United States
Date Requested: 12/10/2016

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