Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
GBE1 is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle.
Immunohistochemical staining of paraffin-embedded colon tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).
Anti-GBE1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1.
Immunofluorescent staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GBE1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
Flow cytometric analysis of Jurkat cells, using anti-GBE1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
HEK293T cells transfected with either pCMV6-ENTRY GBE1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-GBE1 mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.