Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
GATA-4 is a zinc finger transcription factor that is essential for heart development. In mouse heart development, the first expression of GATA-4 is seen in coelomic epithelial cell of the primitive streak (7-7.5 days). During formation and bending of the heart tube, expression is found in endocardium, myocardium and embryonic structures containing precardiac mesoderm. By the onset of cardiac septation (9 days) expression is present in endocardium, endocardial cushion tissue and myocardium.
Anti-GATA4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7989 dilution 10 ug/ml.
Anti-GATA4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7989 dilution 10 ug/ml.
Western blot of GATA-4 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using LS-B7989 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.