Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
GATA-4 is a zinc finger transcription factor that is essential for heart development. In mouse heart development, the first expression of GATA-4 is seen in coelomic epithelial cell of the primitive streak (7-7.5 days). During formation and bending of the heart tube, expression is found in endocardium, myocardium and embryonic structures containing precardiac mesoderm. By the onset of cardiac septation (9 days) expression is present in endocardium, endocardial cushion tissue and myocardium.
Western blot of GATA4 (arrow) using rabbit polyclonal GATA4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2) (Origene Technologies).
Western blot of anti-GATA4 Antibody in CEM cell line lysates (35 ug/lane). GATA4(arrow) was detected using the purified antibody.
GATA4 Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Requested From: United States
Date Requested: 2/25/2017