LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
GAPDH Antibody (clone 2D4A7) IHC‑plus™ LS‑B520
Note: This antibody replaces LS-C2676
GAPDH antibody LS-B520 is an unconjugated mouse monoclonal antibody to GAPDH from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Catalog
Size
Price
LS-B520-100
100 µl (1 mg/ml)
Unavailable

Popular GAPDH Products

Western blot analysis of cell lysates using GAPDH Antibody, pAb, Goat The signal was developed with Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor TM HRP Substrate Kit. Predicted Size: 36 kD Observed Size: 36 kD
Species: Human, Mouse, Rat, Bovine, Goat, Hamster, Pig, Chicken, Fish, E. coli
Applications: Western blot, ELISA
Anti-GAPDH antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2517 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-GAPDH antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2536 concentration 5 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Anti-GAPDH antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8020 dilution 10 ug/ml.
Species: Human, Mouse, Rat, Bovine, Cat, Dog, Goat, Pig, Rabbit, Fish
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: Immunoprecipitation

Product Description

GAPDH antibody LS-B520 is an unconjugated mouse monoclonal antibody to GAPDH from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
About GAPDH
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. P04406 NM_002046 NP_002037.2

GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody


Specifications

Target
Human GAPDH
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [2D4A7]
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:4000)
  • Western blot (1:20000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GAPDH antibody was raised against ni-NTA purified recombinant human GAPDH expressed in E. coli strain BL21 (DE3)
Specificity
GAPDH (2D4A7).
Usage
Immunohistochemistry: LS-B520 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B520 was determined to be 1:4000.
Presentation
Tris-glycine buffer, 150 mM sodium chloride, 0.05% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (2)

1
Apoptosis inhibition or inflammation: the role of NAIP protein expression in Hodgkin and non-Hodgkin lymphomas compared to non-neoplastic lymph node. Mazrouei S, Ziaei A, Tanhaee AP, Keyhanian K, Esmaeili M, Baradaran A, Salehi M. PloS one. 2014 9:4. [Full Text Article] [PubMed:22357131] [PMC:PMC3297494]
2
Livin, a novel marker in lymphoma type distinction. Tanhaei AP, Ziaei A, Mazrouei S, Keyhanian K, Salehi M. Annals of diagnostic pathology. 2014 Jun;18:157-62. [Full Text Article] [PubMed:24767895]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-GAPDH antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B520 dilution 1:4000.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-GAPDH antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B520 dilution 1:4000. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-GAPDH antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B520 dilution 1:4000.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-GAPDH antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B520 dilution 1:4000. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-GAPDH antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B520 dilution 1:4000.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-GAPDH antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B520 dilution 1:4000. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-GAPDH antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B520 dilution 1:4000.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-GAPDH antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B520 dilution 1:4000. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number