(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
Protein G purified
- IHC - Paraffin (1:4000)
- Western blot (1:20000)
- (applications tested for the base form of this product only)
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including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
GAPDH antibody was raised against ni-NTA purified recombinant human GAPDH expressed in E. coli strain BL21 (DE3)
Immunohistochemistry: LS-B520 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B520 was determined to be 1:4000.
Tris-glycine buffer, 150 mM sodium chloride, 0.05% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.