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GAPDH antibody LS-C169552 is an unconjugated rabbit polyclonal antibody to GAPDH from human, mouse, rat and other species. Validated for WB.
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![Western blot of cell lysates using GAPDH Antibody, pAb, Goat (LS-C51084, 0.1 ug/ml) The signal was developed with Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit. Predicted Size: 36 kD Observed Size: 36 kD](https://lsbio-7d62.kxcdn.com//image2/gapdh-antibody-ls-c51084/127901_732269.jpg)
Species:
Human, Mouse, Rat, Bovine, Goat, Hamster, Pig, Chicken, Fish, E. coli
Applications:
Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Bovine, Cat, Dog, Goat, Pig, Rabbit, Fish
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse, Rat
Applications:
Immunoprecipitation
Product Description
GAPDH antibody LS-C169552 is an unconjugated rabbit polyclonal antibody to GAPDH from human, mouse, rat and other species. Validated for WB.
About GAPDH
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton.
P04406
NM_002046
NP_002037.2
GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody
Specifications
Target
Mouse GAPDH
Host
Rabbit
Reactivity
Mouse, Human, Monkey, Rat, Guinea pig, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot
- (applications tested for the base form of this product only)
Immunogen
GAPDH antibody was raised against synthetic peptide from C-Terminus of mouse Gapdh (P16858, NP_032110). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Rabbit, Horse, Guinea pig (100%); Galago, Dog, Bovine, Opossum, Zebra finch, Xenopus, Pufferfish, Nematode (92%).
Blocking Peptide
LS-E14623 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C169552. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C169552. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Mouse GAPDH
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Customer Reviews (0)
Images
Western blot
This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From: United States
Date Requested: 8/15/2018
Date Requested: 8/15/2018
