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GAPDH Antibody (C‑Terminus) LS‑C169552
GAPDH antibody LS-C169552 is an unconjugated rabbit polyclonal antibody to GAPDH from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C169552-50
50 µg
$365

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Species: Human, Mouse, Rat, Bovine, Goat, Hamster, Pig, Chicken, Fish, E. coli
Applications: Western blot, ELISA
Anti-GAPDH antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2517 concentration 5 ug/ml.
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Anti-GAPDH antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2536 concentration 5 ug/ml.
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Anti-GAPDH antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8020 dilution 10 ug/ml.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: Immunoprecipitation

Product Description

GAPDH antibody LS-C169552 is an unconjugated rabbit polyclonal antibody to GAPDH from human, mouse, rat and other species. Validated for WB.
About GAPDH
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. P04406 NM_002046 NP_002037.2

GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody


Specifications

Target
Mouse GAPDH
Host
Rabbit
Reactivity
Mouse, Human, Monkey, Rat, Guinea pig, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
GAPDH antibody was raised against synthetic peptide from C-Terminus of mouse Gapdh (P16858, NP_032110). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Rabbit, Horse, Guinea pig (100%); Galago, Dog, Bovine, Opossum, Zebra finch, Xenopus, Pufferfish, Nematode (92%).
Blocking Peptide
LS-E14623 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C169552. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Mouse GAPDH
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

 This image was taken for the unconjugated form of this product. Other forms have not been tested.
This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

 This image was taken for the unconjugated form of this product. Other forms have not been tested.
This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

 This image was taken for the unconjugated form of this product. Other forms have not been tested.
This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

 This image was taken for the unconjugated form of this product. Other forms have not been tested.
This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 8/15/2018

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