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Anti-GAPDH Antibody (C-Terminus) LS-C169552

Catalog	Size	Price
LS-C169552-50	50 µg (1 mg/ml)	Unavailable

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Antibody:	Rabbit Polyclonal to Mouse GAPDH
Reactivity:	Mouse, Human, Monkey, Rat, Guinea pig, Horse, Rabbit
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Mouse GAPDH
Host:	Rabbit
Reactivity:	Mouse, Human, Monkey, Rat, Guinea pig, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted:	Bovine, Dog, Xenopus (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

GAPDH antibody was raised against synthetic peptide from C-Terminus of mouse Gapdh (P16858, NP_032110). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Rabbit, Horse, Guinea pig (100%); Galago, Dog, Bovine, Opossum, Zebra finch, Xenopus, Pufferfish, Nematode (92%).

Blocking Peptide:

LS-E14623 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C169552. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

C-Terminus

Specificity:

Mouse GAPDH

Packaging

Presentation:	Lyophilized from PBS
Reconstitution:	50 µl Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About GAPDH

Uniprot: P04406 NCBI: NM_002046 NP_002037.2

Synonyms: GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody

Description: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton.

(More About GAPDH)

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Western blot

This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 6/25/2017

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