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Anti-GAPDH Antibody (aa150-200, DY488) LS-C142989


Wt. Vol. Conc. Price
- 100 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Alternate Forms of this Antibody

LS-C49293 Unconjugated
LS-C142987 Biotin Conjugated
LS-C142988 DY650 Conjugated
LS-C142990 DY550 Conjugated

Most Popular GAPDH Antibodies

Anti-GAPDH Antibody (aa30-44, Magnetic beads) LS-C172169
Rabbit Polyclonal to Human GAPDH
Human, Mouse, Rat
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation
Magnetic beads Conjugated
Immunohistochemistry Image

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Rabbit Polyclonal to Human GAPDH
Human, Mouse
ICC, Immunofluorescence, Western blot (applications tested for the base form of this product only)
DY488 Conjugated


Human, Mouse (tested or 100% immunogen sequence identity)
DyLight 488
Immunoaffinity purified


  • ICC (1:200)
  • Immunofluorescence (1:200)
  • Western blot (1:2000 - 1:10000)
  • (applications tested for the base form of this product only)

Specificity and Use

GAPDH antibody was raised against the epitope recognized by this antibody maps to a region between residues 150 and 200 of human Glyceraldehyde-3-Phosphate Dehydrogenase using the numbering given in entry NP_002037.2 (GeneID 2597)
The applications listed have been tested for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.


50 mM sodium borate
Store at 4°C. Do not freeze.
For research use only.


P04406 NM_002046 NP_002037.2

GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton.

Western blot

Western blot
Detection of Human and Mouse GAPDH by Western Blot. Samples: Whole cell lysate from mouse NIH3T3 (5, 15 and 50 mcg) and human HeLa (H; 50 mcg) cells. Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.

Requested From: United States
Date Requested: 10/25/2016

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