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Anti-GAPDH Antibody (aa107-156) IHC-plus™ LS-B10309

Note: This antibody replaces LS-C110488
Catalog Size Price
LS-B10309-50 50 µg Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human GAPDH
Human, Guinea pig, Horse
IHC - Paraffin, Western blot (applications tested for the base form of this product only)


Human, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Dog, Pig, Rabbit (at least 90% immunogen sequence identity)
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified


  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

GAPDH antibody was raised against synthetic peptide located between aa107-156 of human GAPDH (P04406, NP_002037). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Horse, Guinea pig (100%); Orangutan, Gibbon, Baboon, Marmoset, Mouse, Rat, Ferret, Elephant, Panda, Dog, Cat, Rabbit, Pig, Opossum (92%); Hamster, Turkey, Chicken, Armadillo, Salmon (85%).
LS-E14614 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B10309. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.


Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.


P04406 NM_002046 NP_002037.2

GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton.


Anti-GAPDH antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10309 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
GAPDH antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 3/17/2018

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