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Anti-GAP43 Antibody (aa216-226, clone 1.00E+03) LS-C204523

Note: This antibody replaces LS-C180213

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GAP43 Antibodies

Anti-GAP43 Antibody (aa216-226) IHC-plus™ LS-B516
Rabbit Polyclonal to Mouse GAP43
Mouse, Human, Rat
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GAP43 Antibody IHC-plus™ LS-B2157
Mouse Monoclonal (IgG2a,k) to Rat GAP43
Rat, Human, Mouse, Rabbit, Chicken, Snake
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GAP43 Antibody (phospho-Ser41) LS-C94168
Sheep Polyclonal (IgG) to Human GAP43
Human, Mouse, Rat
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1.00E+03] (IgG1,k) to Rat GAP43
Rat, Mouse
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Rat GAP43
Mouse
Rat, Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1.00E+03]
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:10000)

Specificity and Use

GAP43 antibody was raised against c-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, to which an N-terminal Cysteine residue was added to allow chemical coupling to KLH carrier protein. Percent identity by BLAST analysis: Mouse, Rat (100%); Human, Chimpanzee, Rabbit (82%).
aa216-226
Mouse and rat GAP43.
The antibody solution can be used at dilutions of at least 1:1000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.

Packaging

PBS, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About GAP43

P17677 NM_002045 NP_002036.1

GAP43 Antibody, Axonal membrane protein GAP-43 Antibody, B-50 Antibody, Growth associated protein 43 Antibody, Growth-associated protein 43 Antibody, Neural phosphoprotein B-50 Antibody, Neuromodulin Antibody, PP46 Antibody, GAP-43 Antibody, Protein F1 Antibody

This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

Immunocytochemistry

Immunocytochemistry
Mixed neuronal cultures stained with GAP43 antibody (green), RPCA-MAP2, a rabbit antibody to microtubule associated protein 2 (MAP2, red) and DNA (blue). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.

Western blot

Western blot
Western blot of whole rat spinal cord lysates probed with GAP43 antibody antibody to GAP43 in lane 16 and another similar antibody in lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. Note that the strong single band running at about 43kDa corresponds to GAP43.

Requested From: United States
Date Requested: 12/8/2016

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