Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC - Frozen
Specificity and Use
Ganglioside GD3 antibody was raised against fM9 melanoma cells
Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells
Immunohistochemistry (frozen tissue sections). Immunofluorescence of cells in culture. Flow cytometry: 1-2 g per 10E6 cells. Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985). Induction of antibody dependent cellular
0.02 M phosphate buffer, pH 7.6, 0.25 M sodium chloride, 0.1% sodium azide