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Anti-GAL4 / Galectin 4 Antibody (N-Terminus) LS-C23784

Ordering

Wt. Vol. Conc. Price
- 250 µl - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular GAL4 / Galectin 4 Antibodies

Anti-GAL4 / Galectin 4 Antibody (aa77-106, PE) LS-C261846
Rabbit Polyclonal (IgG) to Human GAL4 / Galectin 4
Human
Western blot, Flow Cytometry, ELISA
PE Conjugated
Anti-GAL4 / Galectin 4 Antibody (aa1-326, Biotin) LS-C418831
Rabbit Polyclonal (IgG) to Mouse GAL4 / Galectin 4
Mouse
IHC - Paraffin, IHC - Frozen, ICC, Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Yeast GAL4 / Galectin 4
Yeast
Western blot, Immunoprecipitation
Unconjugated

Details

Yeast GAL4 / Galectin 4
Rabbit
Yeast (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • Western blot (1:500 - 1:2500)
  • Immunoprecipitation (1:500)

Specificity and Use

GAL4 / Galectin 4 antibody was raised against the GAL4 amino terminal fragment of yeast, containing only the DNA-binding domain (1-147) expressed in E. coli. The fragment was purified by PEI (polyethyleneimine cellulose), ammonium sulfate precipitation, heparin agarose and DNA-cellulose.
N-Terminus
Recognizes the DNA-binding domain of GAL4, residues 1-147. Recognize both phosphorylated and non-phosphorylated GAL4. Species cross-reactivity: yeast.
Suitable for use in Western Blot and Immunoprecipitation. However, depending on the sample, additional bands may be observed. Immunoprecipitation: 1:500. Immunoprecipitates GAL4 constructs from 35S-methionine labeled yeast and E. coli. Depending on the nature of the construct, phosphorylated and unphosphorylated forms of GAL4 may also be immunoprecipitated.

Packaging

10 mM sodium phosphate, 10 mM Tris, pH 7.4, dialyzed against PBS, 40% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GAL4 / Galectin 4

P56470 NM_006149 NP_006140.1

LGALS4 Antibody, Antigen NY-CO-27 Antibody, GAL4 Antibody, Galectin 4 Antibody, L36LBP Antibody, L-36 lactose-binding protein Antibody, Gal-4 Antibody, Galectin-4 Antibody, Lactose-binding lectin 4 Antibody

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS4 is an S-type lectin that is strongly underexpressed in colorectal cancer. The 323-amino acid LGALS4 protein contains 2 homologous, approximately 150-amino acid carbohydrate recognition domains and all amino acids typically conserved in galectins.

Requested From: United States
Date Requested: 12/7/2016

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