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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GAD65 Antibody (N‑Terminus) LS‑C15426
GAD65 antibody LS-C15426 is an unconjugated mouse monoclonal antibody to GAD65 from human, mouse and rat. Validated for ELISA, IP and WB.
Antibody
Catalog
Size
Price
LS-C15426-50
50 µl
Unavailable

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Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

GAD65 antibody LS-C15426 is an unconjugated mouse monoclonal antibody to GAD65 from human, mouse and rat. Validated for ELISA, IP and WB.
About GAD65
GAD65 is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Q05329 NM_000818 NP_000809.1

GAD2 Antibody, GAD-65 Antibody, Glutamate decarboxylase 2 Antibody, GAD65 Antibody


Specifications

Target
Rat GAD65
Host
Mouse
Reactivity
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:5000)
  • Immunoprecipitation
  • ELISA (1:10000 - 1:100000)
Immunogen
GAD65 antibody was raised against an 18aa peptide sequence near the N-terminus of rat GAD65.
Epitope
N-Terminus
Specificity
Recognizes rat Glutamate Decarboxylase 65 (GAD65). Species sequence Homology: porcine: 100%; mouse: 94%; human and monkey: 83% (16/18aa).
Usage
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Western Blot: 1:1000-1:5000. ~65kD band in rat brain. ELISA: 1:10000-1:100000 using 50-100 ng UCP2 control peptide/well. Immunoprecipitation: 10ul.
Presentation
100 mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 7/17/2018

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