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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GAD65 Antibody LS-C109497

GAD65 antibody LS-C109497 is an unconjugated rabbit polyclonal antibody to GAD65 from human, mouse and rat. Validated for ICC, IF and WB.
Catalog
Size
Price
LS-C109497-100
100 µl (0.93 mg/ml)
$355
Description
GAD65 antibody LS-C109497 is an unconjugated rabbit polyclonal antibody to GAD65 from human, mouse and rat. Validated for ICC, IF and WB.
Target
Human GAD65
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:500 - 1:3000)
Immunogen
GAD65 antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 353 and 556 of GAD65.
Specificity
Human GAD65. Predicted cross-reactivity based on amino acid sequence homology: mouse (96%), rat (96%), dogs (96%), pig (95%), zebrafish (86%).
Presentation
PBS, 20% glycerol, 0.25% Proclin-300
Storage
Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GAD65
Q05329 NM_000818 NP_000809.1

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Images

Immunofluorescence

Immunofluorescence of paraformaldehyde-fixed HeLa, using GAD65 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa, using GAD65 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. 7.5% SDS PAGE. GAD2 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. 7.5% SDS PAGE. GAD2 antibody diluted at 1:1000.

Immunofluorescence

Immunofluorescence of paraformaldehyde-fixed HeLa, using GAD65 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa, using GAD65 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. 7.5% SDS PAGE. GAD2 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. 7.5% SDS PAGE. GAD2 antibody diluted at 1:1000.

Immunofluorescence

Immunofluorescence of paraformaldehyde-fixed HeLa, using GAD65 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa, using GAD65 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. 7.5% SDS PAGE. GAD2 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. 7.5% SDS PAGE. GAD2 antibody diluted at 1:1000.

Immunofluorescence

Immunofluorescence of paraformaldehyde-fixed HeLa, using GAD65 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa, using GAD65 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. 7.5% SDS PAGE. GAD2 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. 7.5% SDS PAGE. GAD2 antibody diluted at 1:1000.

Requested From: United States
Date Requested: 5/23/2019
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